Which of the following best describes anaphase in meiosis II?

Anaphase in meiosis II is characterized by the separation of sister chromatids. During this phase, the sister chromatids, which were previously held together at their centromeres, are pulled apart by the spindle fibers toward opposite poles of the cell. This separation is essential for ensuring that each daughter cell ends up with a complete set of chromosomes after cytokinesis, ultimately leading to four haploid cells from the original diploid cell.

The other options describe processes that occur during different stages of meiosis or are not specific to anaphase II. For example, the alignment of homologous chromosomes occurs during metaphase I, while chromosome condensation is a feature of prophase I and prophase II. The reformation of nuclear membranes happens during telophase and not specifically during anaphase. Hence, the correct identification of anaphase in meiosis II as the phase where sister chromatids are separated is crucial for understanding the overall process of meiosis and gamete formation.